A new automated cell counter for mammalian cell culture assessment
This paper focuses on a new automated cell counter, the 'Countstar' as seen in figure 1, which uses brightfield image analysis and the trypan blue dye-exclusion method. This has the advantage of automating the process to remove human error and subjectivity, while retaining the standard trypan blue dye-exclusion method. The Countstar instrument uses individually packaged disposable plastic slides and associated software to analyse 20 μl samples. Each slide contains five separate chambers to lower costs and waste. Once a sample is loaded and the cells have settled, the instrument takes around 10 seconds to analyse an image. The Countstar requires no regular maintenance and will save vital time in the laboratory, while reducing human error associated with manual cell counts.